| 质粒名称: | pHAGE NFkB-TA-LUC-UBC-GFP-W |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 49343 |
| 存储实验室: | Darrell Kotton |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 空载体大小: | 4874 bp |
| 完整质粒大小: | 8772bp |
| 载体类型: | Lentiviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称1: | eGFP |
| 插入大小: | 720 bp |
| 插入基因名称2: | Luciferase |
| 插入大小: | 1653bp |
| 插入基因名称3: | NFkB consensus binding sequence |
| 插入大小: | 66bp |
| 插入基因名称4: | TA promoter |
| 插入大小: | 7bp |
| 插入基因名称5: | ubiquitin-C promoter |
| 插入大小: | 411bp |
|
文献引用方法 |
|
| 材料和方法部分: | pHAGE NFkB-TA-LUC-UBC-GFP-W was a gift from Darrell Kotton (Addgene plasmid # 49343 ; http://n2t.net/addgene:49343 ; RRID:Addgene_49343) |
| 参考文献部分: | Lentiviral delivery of RNAi for in vivo lineage-specific modulation of gene expression in mouse lung macrophages. Wilson AA, Kwok LW, Porter EL, Payne JG, McElroy GS, Ohle SJ, Greenhill SR, Blahna MT, Yamamoto K, Jean JC, Mizgerd JP, Kotton DN. Mol Ther. 2013 Apr;21(4):825-33. doi: 10.1038/mt.2013.19. Epub 2013 Feb 12. 10.1038/mt.2013.19 PubMed 23403494 |
买家导航
pHAGE NFkB-TA-LUC-UBC-GFP-W载体基本信息
pHAGE NFkB-TA-LUC-UBC-GFP-W载体图谱质粒图谱和多克隆位点信息
pHAGE NFkB-TA-LUC-UBC-GFP-W载体简介
pHAGE NFkB-TA-LUC-UBC-GFP-W载体序列
LOCUS pHAGE NFkB-TA-LUC-UBC-GFP-W 8772 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION pHAGE NFkB-TA-LUC-UBC-GFP-W .
ACCESSION .
VERSION .
KEYWORDS pHAGE NFkB-TA-LUC-UBC-GFP-W
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8772)
AUTHORS Wilson AA, Kwok LW, Porter EL, Payne JG, McElroy GS, Ohle SJ,
Greenhill SR, Blahna MT, Yamamoto K, Jean JC, Mizgerd JP, Kotton DN
TITLE Lentiviral delivery of RNAi for in vivo lineage-specific modulation
of gene expression in mouse lung macrophages.
JOURNAL Mol Ther. 2013 Apr;21(4):825-33. doi: 10.1038/mt.2013.19. Epub 2013
Feb 12.
PUBMED 23403494
REFERENCE 2 (bases 1 to 8772)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8772
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 52..71
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 263..896
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 943..1068
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1565..1798
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1983..2027
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2320..2437
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2532..2563
/label=minP
/note="minimal TATA-box promoter with low basal activity"
regulatory 2619..2628
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2625..4277
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(2691..2709)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
promoter 4284..4682
/label=UbC promoter
/note="human ubiquitin C promoter"
CDS 4691..5410
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 4691..5407
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4736..4757)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4997..5016)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5344..5365
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 5424..6012
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5477..5497)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5895..5906)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6087..6320
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(6439..6457)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6525..6629
/gene="bla"
/label=AmpR promoter
CDS 6630..7490
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6848..6867)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7661..8249
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8150..8169
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8403..8420
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 8512..8531
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 8660..8681
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8696..8726
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8734..8750
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8739..8761
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 8758..2
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8758..2
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
ORIGIN
1 acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
61 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
121 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
181 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
241 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
301 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
361 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
421 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
481 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
541 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
601 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
661 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
721 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
781 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
841 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
901 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
961 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
1021 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
1081 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
1141 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
1201 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
1261 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
1321 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
1381 aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag
1441 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
1501 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
1561 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
1621 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
1681 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
1741 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
1801 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
1861 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
1921 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
1981 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
2041 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
2101 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
2161 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
2221 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
2281 ggtatcgccg aattcacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2341 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2401 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2461 agatccagtt tggactagtg ggaatttccg ggaatttccg ggaatttccg ggaatttcca
2521 gatctagact ctagagggta tataatggaa gctcgaattc cagcttggca ttccggtact
2581 gttggtaaaa agcttggcat tccggtactg ttggtaaagc caccatggaa gacgccaaaa
2641 acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac
2701 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
2761 atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag
2821 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
2881 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
2941 acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg
3001 tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca
3061 tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
3121 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct
3181 tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta
3241 aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta
3301 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
3361 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
3421 atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc
3481 tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt
3541 tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag
3601 cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta
3661 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
3721 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
3781 aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg
3841 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
3901 gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag
3961 gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg
4021 acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg
4081 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
4141 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
4201 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
4261 gaaagatcgc cgtgtaagga tccggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
4321 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
4381 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
4441 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
4501 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
4561 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
4621 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
4681 ggccgcccat atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
4741 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
4801 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
4861 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
4921 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
4981 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
5041 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
5101 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
5161 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
5221 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
5281 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
5341 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
5401 gtacaagtaa atcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga
5461 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
5521 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
5581 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
5641 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
5701 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
5761 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
5821 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
5881 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
5941 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
6001 ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg tagatcttag
6061 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
6121 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
6181 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
6241 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
6301 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
6361 tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc
6421 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
6481 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
6541 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
6601 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
6661 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
6721 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
6781 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
6841 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
6901 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
6961 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
7021 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
7081 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
7141 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
7201 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
7261 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
7321 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
7381 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
7441 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
7501 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
7561 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
7621 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
7681 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
7741 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
7801 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
7861 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
7921 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
7981 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
8041 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
8101 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
8161 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
8221 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
8281 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
8341 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
8401 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
8461 cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc
8521 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
8581 aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg
8641 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
8701 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
8761 gaaacagcta tg
//
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pHAGE NFkB-TA-LUC-UBC-GFP-W载体质粒应用举例
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